Coordinator: Luísa Barreira (email@example.com)
We often work in a chemistry lab to do simple things as measuring a pH or the salinity of seawater. Or some more complicated stuff such as determining the protein concentration or the chlorophyll content of microalgae or even better: the fatty acid profile of a seaweed. All of these analyses require the use of a particular equipment: a pH meter, a spectrophotometer, GC-MS. Even though you sometimes work with this sort of equipment, do you really understand what is behind the numbers that they give you? Can you tell if a pH meter is properly calibrated? Do you know the difference between UV-vis absorbance and a fluorescence measurement? Are you planning on using AAS, HPLC, GC or some other techniques? Then this is the curricular unit for you.
The objectives of this course are: i) explain the basic concepts behind the different instrumental techniques that we often use in a laboratory to analyse and characterize biological samples (bio)chemically; 2) give you a glimpse of what you can do with them and how they can help you in your MSc thesis; and teach you how to correctly operate these equipment to ensure that you can trust your results. In this course we will work with potentiometry (e.g., pH measurement), spectrophotometry, spectrofluorimetry (e.g., fluorescence), atomic spectrophotometry, and both liquid and gas chromatography.
30 h of lectures + 40 h of labs + 98 h of independent work.
The student will use the time assigned as "independent work" to study for a written exam and write two reports.
As the registration for any elective is mandatory, please wait for an email by the MSc Coordinating Team requesting the names of the electives that you plan to choose for your 2nd year, 1st semester.
Final grade: 70% of theoretical assessment via a written exam + 30% of practical assessment. The latter grade will be the average of the grades obtained in 3 reports.